Resources for learning more#

Resource Name

Link

Brief description

🌐 Microforum

link

An online forum for discussing issues with and getting advice on sample preparation and microscopy

🌐 FPbase 13

link

Database for identifying fluorophores by brightness, spectra and assessing compatibility with other fluorophores and with microscope filters

🌐 Bio-protocol

link

Website to search for protocols across biological disciplines, including protocols associated with work published elsewhere. All protocols are available under an open access license (CC BY or CC BY-NC)

🌐 protocols.io

link

A secure platform for developing and sharing reproducible methods

📄 Designing a rigorous microscopy experiment: Validating methods and avoiding bias 9

link

Review of aspects of designing a rigorous light microscopy experiment, including validation of samples and imaging, identification and correction of errors, and strategies to avoid biases

📄 Tutorial: guidance for quantitative confocal microscopy 5

link

Step-by-step guidance on practical considerations for sample preparation, acquisition, and image analysis; primarily though not exclusively aimed at users of confocal microscopy